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| 基因名 | FEN1 |
| 英文全称 | flap structure-specific endonuclease 1 |
| 别称 | DNase IV, FEN 1, FEN1, Flap endonuclease 1, flap structure endonuclease 1, hFEN 1, Maturation factor 1, MF1, RAD2 |
| 中文名称 | 瓣结构特异性核酸内切酶1 |
| Uniprot | P39748 |
| GeneID | 2237 |
| 交叉反应(推测) | Human Mouse Rat (Bovine Zebrafish Fruit fly Chicken Sheep) |
| 氨基酸大小 | 43kDa |
| 表达氨基酸 | 1-380aa |
| Background | The protein encoded by this gene removes 5 overhanging flaps in DNA repair and processes the 5 ends of Okazaki fragments in lagging strand DNA synthesis.Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another.The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication.DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5 end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene.Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. |
| Application | Immunology research |
| Derived from | E.coli |
| Purification | NI-NTA affinity purification |
| Formulation | Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0. |
| Shipping | The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| Storage | Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
| Reconstitution | Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | Purity≥85% as determined by reducing SDS-PAGE. |
| 规格 | 10ug 50ug 100ug 1mg |