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| 产品名称： | pSTMV5 |
|---|---|
| 商品货号： | TS131923 |
| Designations： | pSTMV5 |
| GenBank Number： | M25782 |
| Species： | satellite tobacco mosaic tobamovirus (STMV) |
| Depositors： | G Kurath |
| Applications： | Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand. |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.059000015258789 |
| Insert Size (kb)： | 1.059 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI/SphI--3.4, 1.15; SalI--4.4; BglII--4.4. The insert contains the following restriction sites (bp from the 5 XbaI site): DraI--321; SalI--602; BglII--791. The primer-derived NsiI site is at nt 1059. Clones pSTMV5 and pSTMV6 (TS131923, 45136) can be distinguished by RNase protection assays which give distinct patterns due to an A-C difference at nt 751 of the STMV sequence. The RNA transcribed from this clone is infectious. Contains a complete STMV genome representing the major genome type within the STMV type strain. pSTMV5 is identical to the reported STMV type strain sequence except for a G to A difference at nt 494. Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand. Constructed using primers for cDNA synthesis that added NsiI and SphI sites at the 3 end and an XbaI site at the 5 end. |
| Classification： | Tobamovirus |
| References： | Kurath G, et al. Analysis of genetic heterogeneity within the type strain of satellite tobacco mosaic virus reveals several variants and a strong bias for G to A substitution mutations. Virology 189: 233-244, 1992. PubMed: 1604813 Mirkov TE, et al. Nucleotide sequence and translation of satellite tobacco mosaic virus RNA. Virology 170: 139-146, 1989. PubMed: 2718378 Gael Kurath, personal communication |