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| 产品名称： | pUCSVH388-2 molecular clone of Simian virus 40, SV-40 |
|---|---|
| 商品货号： | TS131935 |
| Designations： | pUCSVH388-2 molecular clone of Simian virus 40, SV-40 |
| Species： | Simian virus 40, Simian virus 40 |
| Depositors： | JA Lednicky, JS Butel |
| Isolation： | Description of cloned agent: Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp. High-copy number; pUC-19 has a ColEI ori. |
| Applications： | contains sequence |
| Vector： | Construct size (kb): 7.914999961853027 DESCRIPTION OF VECTOR COMPONENT xa0xa0xa0xa0xa0xa0xa0 Type of vector:xa0 E. coli xa0xa0xa0xa0xa0xa0xa0 Name of vector:xa0 pUC-19 xa0xa0xa0xa0xa0xa0xa0 Intact vector size (kb):xa0 2.686 kb |
| Insert： | DNA: genomic Insert lengths(kb): 5.229000091552734 Tissue: monkey brain |
| Insert Size (kb)： | 5.229 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Description of cloned agent: Name: SV40 Strain: H388, variant #2 Classification: Papovavirus Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp. High-copy number; pUC-19 has a ColEI ori. VRMC-10 (variant #2) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-11 and VRMC-9). Restriction enzyme analysis: BamHI--4.501,3.414; EcoRI--5.229,2.686; KpnI--6.414,1.501; SalI--7.915; SfiI--7.915; XhoI--uncut. Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by J. Lednicky. Viable clone; verif. by lipofection of EcoRI-linearized DNA into CV-1 cells (ATCC CCL-70, passage 35) by J. Lednicky (lipofection date: 1/12/97). |
| Classification： | Polyomaviridae, Polyomavirus |
| References： | Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685 John A Lednicky, personal communication |