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| 产品名称： | pIB16.12 |
|---|---|
| 商品货号： | TS132000 |
| Designations： | pIB16.12 |
| GenBank Number： | M63838 |
| Species： | Homo sapiens, human |
| Depositors： | JA Trapani |
| Vector： | Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSP65 Intact vector size: 3.010 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: SP6 replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IFI16 Other gene symbols: IFNFIP1 Genomic copy number: unique Gene name: interferon, gamma-inducible protein 16 Contains complete coding sequence?: U Chromosome: 1; Localization: 1 q22 Tissue: T lymphocyte, activated Type of DNA: cDNA Insert 5 end: Modification: linker Insert 3 end: Insert size (kb): 1.53 Cross references: DNA Seq. Acc.: M63838 Insert lengths(kb): 1.529999971389771 Tissue: T lymphocyte, activated Gene product: interferon, gamma-inducible protein 16(interferon gamma-inducible protein 16) IFI16 |
| Insert Size (kb)： | 1.530 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; HindIII--3.4, 1.1; PstI--3.0, 1.5; PvuII--2.5, 1.2, 0.7; SacI--4.5. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.32; HindIII--0.79; PvuII--1.29. Corresponds to nt 403-1933 of the published sequence (encoding aa 135-644). Detects a transcript of 2.7 kb in lymphoid cells. |
| References： | Trapani JA, et al. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells. Immunogenetics 36: 369-376, 1992. PubMed: 1526658 |