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微信小章| 产品名称： | pCMV5Asp36 |
|---|---|
| 商品货号： | TS132051 |
| Designations： | pCMV5Asp36 |
| GenBank Number： | J00136 |
| Species： | Homo sapiens, human |
| Depositors： | W Cheung |
| Applications： | in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Vector： | Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCMV5 Intact vector size: 4.700 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI BamHI HindIII Polylinker sites: Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): promoter: CMV, -> terminator: hGH, -> replicon: SV40 replicon: pMB1 marker(s): ampR, replicon: f1, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: F9 Genomic copy number: unique Gene name: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) Contains complete coding sequence?: U Chromosome: X; Localization: X q26.3-q27.1 Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 2.8 Cross references: DNA Seq. Acc.: J00136 Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) F9 Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.5, 2.9; BglII--7.8; EcoRI--4.8, 2.8; NotI--7.8; SstI--7.2, 0.53. Digestion with EcoRI produces fragments of 5.0 and 2.5 kb. Bacteria may grow slowly due to the high plasmid copy number. Encodes the following mutant form: E36D. |
| References： | Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370 Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159 Wing-Fai Cheung, personal communication |