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| 产品名称： | pSCH4500 |
|---|---|
| 商品货号： | TS132053 |
| Designations： | pSCH4500 |
| GenBank Number： | L06156 |
| Species： | Providencia stuartii (Buttiaux et al.) Ewing |
| Depositors： | KJ Shaw |
| Applications： | The 375bp BclI fragment is recommended as a hybridization probe for aac(2)-Ia. |
| Vector： | Construct size (kb): 4.25 |
| Insert： | DNA: genomic Insert lengths(kb): 1.305999994277954 Gene product: aminoglycoside 2-N-acetyltransferase aac(2)-Ia |
| Insert Size (kb)： | 1.306 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV/XhoI--2.9, 1.3; EcoRI--4.3; XhoI--4.3; EcoRI--4.3; HindIII--uncut; BamHI--4.3. The insert contains the following restriction sites (approximate kb from the 5 end): HpaI--0.01; BclI--0.41, 0.79; NdeI--0.46; BstXI--0.53. Two rounds of gel purification of the restriction digest generated probe is necessary. Failure to do so often results in high backgrounds (due to flanking regions in the plasmid insert) and false positives with clinical E. coli strains. The 375bp BclI fragment is recommended as a hybridization probe for aac(2)-Ia. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |