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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS132111 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | TAP56 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | leu thi hsdR rpsL supE44 pro+ nad+ lac(deltaU169) galK(Am) lambdakil cI857deltabioA |
| Type Strain： | no |
| Comments： | Host containing defective prophage for use with vectors regulating expression from the lambda PL promoter. Encodes cI857 and a functional N gene. Phenotype verified: galK(am), strR, leu, thi. Because of the temperature sensitive repressor, heat shock for CaCl2 transformations should be at 32C for 10 min, followed by 1 min on ice. Add LB and grow at room temperature for 1-2 hours. Selection for ampR is on LB + amp (100 ug/ml) plates incubated at 30-32C. An isogenic host without N gene function is TAP106, ATCC 47075. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 30.0°C |
| Name of Depositor： | TA Patterson |
| References： | Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761 Patterson TA, et al. Improved bacterial hosts for regulated expression of genes from lambda PL plasmid vectors. Gene 132: 83-87, 1993. PubMed: 8406046 Thomas A Patterson, personal communication |