产品名称: | pH2L |
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商品货号: | TS132116 |
Designations: | pH2L |
Species: | Homo sapiens, human |
Vector: | Construct size (kb): 3.680000066757202 |
Insert: | DNA: cDNA Insert lengths(kb): 0.7300000190734863 Tissue: erythroleukemic cell line Gene product: glycophorin A (includes MN blood group)(glycophorin A) MNS |
Insert Size (kb): | 0.730 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.75; BamHI--3.8; HindIII--3.8; PstI--3.8; XbaI--3.8. The insert includes the complete coding sequence, extending from 3 bp upstream of the initiation methionine to 276 bp downstream from the stop codon. The nucleotide sequences encoding the NH2-terminal leader peptide and first 26 amino acids of GYPA and GYPB are nearly identical. There is also a very homologous region encoding the presumed membrane-spanning portion of the proteins. This probe detects the same polymorphism as HGpA-3. |
References: | Siebert PD, Fukuda M. Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure. Proc. Natl. Acad. Sci. USA 83: 1665-1669, 1986. PubMed: 3456608 Minoru Fukuda, personal communication |