宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pH2L

货号 TS132116
中文名称 null
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产品简介
购买须知
产品名称: pH2L
商品货号: TS132116
Designations: pH2L
Species: Homo sapiens, human
Vector:
Construct size (kb): 3.680000066757202
Insert:
DNA: cDNA
Insert lengths(kb): 0.7300000190734863
Tissue: erythroleukemic cell line
Gene product: glycophorin A (includes MN blood group)(glycophorin A) MNS
Insert Size (kb): 0.730
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.75; BamHI--3.8; HindIII--3.8; PstI--3.8; XbaI--3.8.
The insert includes the complete coding sequence, extending from 3 bp upstream of the initiation methionine to 276 bp downstream from the stop codon.
The nucleotide sequences encoding the NH2-terminal leader peptide and first 26 amino acids of GYPA and GYPB are nearly identical. There is also a very homologous region encoding the presumed membrane-spanning portion of the proteins.
This probe detects the same polymorphism as HGpA-3.
References:

Siebert PD, Fukuda M. Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure. Proc. Natl. Acad. Sci. USA 83: 1665-1669, 1986. PubMed: 3456608

Minoru Fukuda, personal communication