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微信小章| 产品名称： | pBAD24 in E. coli |
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| 商品货号： | TS132142 |
| Designations： | pBAD24 in E. coli |
| Depositors： | L Guzman, J Beckwith |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.54
Vector: pBAD24
Promoters: Promoter araC
Construction: pBAD22
Marker(s): ampR, araC
Features: initiation codon: ATG
marker(s): ampR operator: I2 + I1 operator: O1 operator: O2 other: CAP site other: Kozak sequence promoter: araC promoter: bla (ampR) promoter for expression: arabinose BAD regulator: araC replicon: M13 replicon: pMB1 MCS: NheI...HindIII ribosome-binding site: Shine-Dalgarno sequence transcription terminator: rrnB T1 + T2 |
| Applications： | expression vector produces protein arabinose regulator vector containing primer sites useful for sequencing |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; BamHI--4.3, 0.1; AvaI--1.8, 1.65, 1.2 Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. The following primers can be used for sequencing of cloned inserts: 5 primer (27 - 8 bp upstream of the NheI site) 5-CTGTTTCTCCATACCCGTT-3; and one of two 3 primers: 3 primer 1 (2 - 19 bp downstream of the HindIII site) 5-CTCATCCGCCAAAACAG-3; 3 primer 2 (17 - 33 bp downstream of the HindIII site) 5-GGCTGAAAATCTTCTCT-3. Vector contains an optimized Shine-Dalgarno ribosome binding site, translation initiation codon and Kozak sequence (relevant for expression in eukaryotic cells) which promote a high level of expression of cloned inserts. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of Escherichia coli containing the plasmid |