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| 产品名称： | CHLA-03-AA |
|---|---|
| 商品货号： | TS132222 |
| Organism： | Homo sapiens, human |
| Tissue： | Brain (Temporal lobe) |
| Product Format： | frozen |
| Morphology： | rounded-floating or loosely attached (neuronal-like) |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | anaplastic astrocytoma |
| Age： | 9 years |
| Gender： | Female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | CHLA-03-AA was established from a 9 year old girl with recurrent anaplastic astrocytoma (AA). Cells were isolated from freshly resected brain tumor and were cultured in neurobasal medium after mechanical disruption. |
| Comments： | Cultures grow as floating aggregates of round cell clusters and as lightly attached neuronal-like cells. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Childrens Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community. |
| Complete Growth Medium： | DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
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| Subculturing： | Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, subculture by gently dislodging the cells in the spent culture medium by pipetting across the monolayer or by rapping the side of the flask sharply with the palm of your hand (the latter is only preferable when working with larger flasks), followed by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. Medium renewal: Add fresh medium twice weekly (depending on cell density). |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11 D13S317: 10, 11 D7S820: 9, 10 D16S539: 11, 12 vWA: 16, 18 TH01: 6, 9 Amelogenin: X TPOX: 8, 9 CSF1PO: 10 |
| Name of Depositor： | A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles |