产品名称: | pKLApoRM11-6 pKLApoIRM11-6 |
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商品货号: | TS132242 |
Designations: | pKLApoRM11-6 pKLApoIRM11-6 |
Species: | Arthrobacter protophormiae (Lysenko) Stackebrandt et al. |
Depositors: | New England Biolabs, Inc. |
Applications: | in suitable host, produces protein modification methylase ApoI in suitable host, produces protein restriction endonuclease ApoI |
Vector: | Construct size (kb): 9.2 |
Insert: | DNA: genomic Insert information: Insert size (kb): 4.8 Genexa0: restriction endonuclease ApoI, modification methylase ApoI DNAxa0: genomic Sourcexa0: Arthrobacter protophormiae ATCC 55228 Insert endsxa0: BglII Insert lengths(kb): 4.80 Gene product: restriction endonuclease ApoI hsdM Target Gene: modification methylase ApoI, restriction endonuclease ApoI |
Insert Size (kb): | 4.800 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Comments: | Restriction digests of the clone give the following sizes (kb): PstI--4.8, 2.5, 1.5, 0.5; HindIII--6.7, 2.5. The insert contains the following restriction sites (approximate kb from one end): PstI--1.25, 1.75, 3.6; BamHI--1.83, 2.6, 2.8, 4.5; HindIII--2.08. Contains and expresses the complete methylase and endonuclease genes. Distributed in aliquots of 200 - 250 ng (20-25 ng/ul). |
References: | Polisson C, et al. Novel type II restriction endonuclease, ApoI, obtainable from Arthrobacter protophormiae and a process for producing the same. US Patent 5,200,337 dated Apr 6 1993 |
Disclosure: | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |