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微信小章| 产品名称： | pB8 lambdaB1 |
|---|---|
| 商品货号： | TS132276 |
| Designations： | pB8 lambdaB1 |
| GenBank Number： | J02775 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.600000023841858 Tissue: hepatocellular carcinoma Hep G2 cell line Gene product: apolipoprotein B (including Ag(x) antigen) APOB Alleles: B1, B3, B5, D2, B2, B4, D1 |
| Insert Size (kb)： | 1.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.2, EcoRI/PstI--2.7, 1.6; EcoRI--4.2; HindIII--3.4, 0.7 doublet; PvuII--2.3, 1.3, 0.67. Detects the hypervariable VNTR RFLP of the AT-rich minisatellite at the 3 end of APOB when genomic DNA is digested with HindIII and MspI but not with XbaI. Insert ends are from linkers and are not present in the cDNA itself. |
| References： | Hegele RA, et al. Apolipoprotein B-gene DNA polymorphisms associated with myocardial infarction. N. Engl. J. Med. 315: 1509-1515, 1986. PubMed: 3024002 Huang LS, et al. Human apolipoprotein B cDNA clone isolation and demonstration that liver apolipoprotein B mRNA is 22 kilobases in length. Proc. Natl. Acad. Sci. USA 82: 6825-6829, 1985. PubMed: 2995989 Huang LS, Breslow JL. A unique AT-rich hypervariable minisatellite 3 to the ApoB gene defines a high information restriction fragment length polymorphism. J. Biol. Chem. 262: 8952-8955, 1987. PubMed: 2885324 Jan L Breslow, personal communication |