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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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lambdaPM-6665 [6665]

货号 TS132289
中文名称 null
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产品名称: lambdaPM-6665 6665
商品货号: TS132289
Designations: lambdaPM-6665 6665
GenBank Number:

U00062

Species: Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors: MV Olson, L Riles
Vector:
Construct size (kb): 0.0
Insert:
DNA: genomic
Insert lengths(kb): 16.64200019836426
Gene product: pyrroline-5-carboxylate dehydrogenase PUT2
Insert Size (kb): 16.642
Related Products:
component of:ATCC 77284
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 0.761, 2.953, 0.927, 0.709, 0.909, 1.013, 0.836, 1.239, 1.921, 0.935, 0.632, 0.828, 0.761, 0.467, 1.675.
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--23.0, 10.0, 7.8, 3.9, 2.9, 1.9, 1.6, 1.2, 1.0; BamHI--25.0, 13.0, 6.8, 5.6; PvuII--23.0, 4.1, 3.9 (doublet), 3.5, 3.3, 2.4, 2.1, 1.6, 1.0, 0.5.
Overlaps clones with ATCC number(s): 70373, 70807, 70831.
Corresponds to nt 171891-188533 of the sequence of chromosome VIII.
Classification: Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References:

Olson MV, et al. Random-clone strategy for genomic restriction mapping in yeast. Proc. Natl. Acad. Sci. USA 83: 7826-7830, 1986. PubMed: 3463999

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Linda Riles, personal communication