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| 产品名称： | Rotavirus A |
|---|---|
| 商品货号： | TS132297 |
| Classification： | Reoviridae, Rotavirus, Rotavirus A |
| Deposited As： | Human rotavirus |
| Agent： | Rotavirus A |
| Strain： | DS-1 |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | human infant, Bethesda, MD |
| Product Format： | frozen |
| Storage Conditions： | -20°C to -70°Cxa0 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | may cause gastroenteritis in infants -20C to -70C Propagate in cells fed with serum-free medium containing 0.5 ug/ml purified trypsin. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (rounding and detachment of cells, plaque formation) in MA-104 cells with massive cell destruction in 5 to 6 days Vague CPE characterized by rounding and clumping of cells. Cell detachment and the appearance of floating cells in 3-6 days. |
| Recommended Host： | MA-104 cells (ATCC CRL-2378) Host of Choice:xa0 MA-104 (CRL-2378) Host Range: MA-104, CV-1 MA-104 cells (ATCC CRL-2378); CV-1 cells (ATCC CCL-70) |
| Growth Conditions： | Temperature: 36.0°C Duration: 1-4 days at 36C |
| Cryopreservation： | Storage temperature: -20C to -70C |
| Name of Depositor： | Childrens Hospital of Philadelphia, Wistar Institute of Anatomy and Biology |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | NCRR Contract |
| Chain of Custody： | ATCC < |
| Source： | human infant, Bethesda, MD |
| References： | Clark HF, et al. Rotavirus reassortant vaccine. US Patent 5,626,851 dated May 6 1997 |