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| 产品名称： | ACY1-E |
|---|---|
| 商品货号： | TS132316 |
| Designations： | ACY1-E |
| Species： | Homo sapiens, human |
| Depositors： | YE Miller |
| Vector： | Construct size (kb): 4.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.384999990463257 Tissue: liver Gene product: aminoacylase I(aminoacylase, aminoacylase 1) ACY1 |
| Insert Size (kb)： | 1.385 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.4; PstI--3.1, 0.8, 0.15; HindIII--4.1; BamHI--4.1; SalI--4.1. The insert contains the following restriction sites (nt from the 5 end): PstI--716, 875; MboI--1025. The insert is oriented in the vector so as to generate a fusion protein. Detects a genomic EcoRI fragment of 22 kb mapping to chromosome 3 and a genomic EcoRI fragment of 4.4 kb mapping to chromosome 18. The latter fragment is less intensely visualized in both EcoRI and KpnI digests. Detects a major 0.9 kb mRNA and a minor 4.2 kb mRNA in human liver, fibroblasts, and a variety of cell lines. Also detects a 0.9 kb mRNA in the K1 Chinese hamster ovary cell line. |
| References： | Miller YE, et al. Human aminoacylase-1: cloning, regional assignment to distal chromosome 3p21.1, and identification of a cross-hybridizing sequence on chromosome 18. Genomics 8: 149-154, 1990. PubMed: 1707030 York E Miller, personal communication |