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| 产品名称： | Rhesus rotavirus (MMU 18006) |
|---|---|
| 商品货号： | TS132363 |
| Classification： | Reoviridae, Rotavirus |
| Agent： | Rhesus rotavirus (MMU 18006) |
| Strain： | S:USA:79:2 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Derived by adaptation of primary cell culture product ATCC VR-954 to MA104.1 (ATCC CRL-2378.1) cells. (Note: ATCC VR-954 and TS132363 have not been compared with respect to sequence or infectivity in primary cell culture and tissue culture). |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Derived by adaptation of primary cell culture product ATCC VR-954 to MA104.1 (ATCC CRL-2378.1) cells. (Note: ATCC VR-954 and TS132363 have not been compared with respect to sequence or infectivity in primary cell culture and tissue culture). |
| Effect on Host： | CPE in the form of refractile, rounded cells throughout monolayer or in loose clusters. CPE in the form of refractile, rounded cells throughout monolayer or in loose clusters. |
| Recommended Host： | Production Host: MA104.1 (ATCC CRL-2378.1) Recommended Host: xa0MA104.1 (ATCC® CRL-2378.1x99) Alternate Hosts: BS-C-1 (ATCC CCL-26), Rhesus embryonic monkey kidney cells, Primary cynomolgus monkey kidney cells |
| Growth Conditions： | For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent, not 100% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubate at 37.0°C in a humidified, 5% CO2 atmosphere for 2-3 days, until CPE are well advanced in 100% of the culture. In TC, ATCC VR-1739 should be grown in serum free media that contains 2.0µg/ml trypsin. |
| Name of Depositor： | RW Emmons and NJ Schmidt |
| Special Collection： | ATCC |
| Source： | Stool sample from Macaca mulatta monkey with diarrhea. This product has been transferred into a different host at ATCC. |