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微信小章| 产品名称： | pha2m1 phigf4 |
|---|---|
| 商品货号： | TS132413 |
| Designations： | pha2m1 phigf4 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.099999904632568 Tissue: adult liver Gene product: alpha-2-macroglobulin A2M Alleles: B2, C2, E1, A2, B1, B1, C1, D1, D2, E2, F1, F2, A1, B2, E1, E2 |
| Insert Size (kb)： | 2.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.8, 1.4, 0.66; EcoRI--5.9; ClaI--4.4, 1.48; AvaI--3.3, 2.6; BamHI--5.9. The insert encodes amino acids 809-1451 of the alpha-2-microglobulin gene. Codominant segregation of the BglII polymorphism demonstrated in 2 families. Codominant segregation of the PvuII polymorphism demonstrated in 5 families. Codominant segregation of the HinfI polymorphism demonstrated in 5 families (35 individuals). Codominant segregation of the PvuII polymorphism demonstrated in 2 families (20 individuals). HaeIII, HinfI and PuvII RFLPs are independent mutations. Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, HincII, RsaI, HindIII, MspI, PstI. Enzyme(s) not detecting polymorphism: AvaII, BamHI, EcoRV, StuI, AluI, NciI, MspI, PstI. Enzyme(s) not detecting polymorphism: HindIII, KpnI, MspI, PstI, SacI, ScaI, XbaI, XmnI. |
| References： | Bell GI, et al. Human alpha2-macroglobulin gene is located on chromosome 12. Somatic Cell Mol. Genet. 11: 285-289, 1985. PubMed: 2408344 Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597 Scambler PJ, et al. Two RFLPs for human alpha-2 macroglobulin (A2M). Nucleic Acids Res. 13: 6787, 1985. PubMed: 2414729 Marynen P, et al. Three RFLPs associated with the human alpha2-macroglobulin gene (A2M). Nucleic Acids Res. 13: 8287, 1985. PubMed: 2415929 Barbara Weiffenbach, personal communication |