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购买须知
| 产品名称： | TIS8H7 |
|---|---|
| 商品货号： | TS132418 |
| Designations： | TIS8H7 |
| Species： | Homo sapiens, human |
| Depositors： | KM Sakamoto |
| Vector： | Construct size (kb): 9.399999618530273 |
| Insert： | DNA: genomic Insert lengths(kb): 6.699999809265137 Tissue: neutrophil Gene product: early growth response 1(krox-24, zif-268) EGR1 |
| Insert Size (kb)： | 6.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--6.8, 2.7; SstI--2.7, 2.2, 1.9, 1.25, 1.1, 0.2; BglII--9.4; PvuII--2.3, 2.1, 1.65, 1.5, 0.78 (doublet); EcoRI--9.4. The insert contains the following restriction fragments (approximate kb from the 5 end): SstI--0.1, 1.14, 2.3, 4.4; BglII--2.8; ApaI--2.0, 2.5, 3.14, 5.3. Contains the complete gene (2 exons and 1 intron) and 5 and 3 flanking sequences. The 500 bp ApaI fragment from the promoter region is sufficient to confer responsiveness to tetradecanoyl phorbol acetate in transient transfection assays. |
| References： | Sakamoto KM, et al. 5 upstream sequence and genomic structure of the human primary response gene, EGR-1/TIS8. Oncogene 6: 867-871, 1991. PubMed: 2052361 |