宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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TIS8H7

货号 TS132418
中文名称 null
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产品简介
购买须知
产品名称: TIS8H7
商品货号: TS132418
Designations: TIS8H7
Species: Homo sapiens, human
Depositors: KM Sakamoto
Vector:
Construct size (kb): 9.399999618530273
Insert:
DNA: genomic
Insert lengths(kb): 6.699999809265137
Tissue: neutrophil
Gene product: early growth response 1(krox-24, zif-268) EGR1
Insert Size (kb): 6.700
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII--6.8, 2.7; SstI--2.7, 2.2, 1.9, 1.25, 1.1, 0.2; BglII--9.4; PvuII--2.3, 2.1, 1.65, 1.5, 0.78 (doublet); EcoRI--9.4.
The insert contains the following restriction fragments (approximate kb from the 5 end): SstI--0.1, 1.14, 2.3, 4.4; BglII--2.8; ApaI--2.0, 2.5, 3.14, 5.3.
Contains the complete gene (2 exons and 1 intron) and 5 and 3 flanking sequences. The 500 bp ApaI fragment from the promoter region is sufficient to confer responsiveness to tetradecanoyl phorbol acetate in transient transfection assays.
References:

Sakamoto KM, et al. 5 upstream sequence and genomic structure of the human primary response gene, EGR-1/TIS8. Oncogene 6: 867-871, 1991. PubMed: 2052361