微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pSV2RbetaG pSV2beta-globin |
|---|---|
| 商品货号： | TS132501 |
| Designations： | pSV2RbetaG pSV2beta-globin |
| Depositors： | P Berg, M Dieckmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.7199997901916500 Vector: pSV2RbetaG (plasmid) Promoters: Promoter SV40 early Marker(s):ampR Construct size (kb): 4.71999979019165 Features: marker(s): ampR promoter: SV40 early replicon: pMB1 terminator: SV40 enhancer: SV40 early |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BglII--4.23, 0.49; PvuII/PstI--2.22, l.55, 0.96; PstI--3.76, 0.96. Expresses beta-globin in mammalian cells. This plasmid includes the SV40 promoter (SV nt 270-5171), rabbit beta globin (487 bp), the SV40 splice site (SV nt4100-4710), the SV40 poly(A) (SV nt 1782-2770) and pBR322 (pBR nt 2067-4362). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549 Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915 Paul Berg, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |