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| 产品名称： | ET1c lambda-EZZ-gamma3 |
|---|---|
| 商品货号： | TS132505 |
| Designations： | ET1c lambda-EZZ-gamma3 |
| Species： | Homo sapiens, human |
| Depositors： | KD Bloch |
| Applications： | For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended. |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Tissue: umbilical vein endothelium, LPS-stimulated Gene product: endothelin 1 EDN1 Alleles: A1, B1, B2, A2, B3, B4 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.9, 0.9; HindIII--4.0, 1.7; SspI--2.3, 2.3, 0.7, 0.3; AvaI--4.3, 1.4; BglI--4.5, 1.2. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.75; EcoRI--1.2; SspI--1.79, 2.04. Because of the internal EcoRI site, the insert cannot be excised intact. For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended. This insert contains two identical copies of 3 0.9 kb EcoRI fragments. Enzyme(s) not detecting polymorphism: AvaII, BamHI, BanII, BclI, BglII, EcoRI, HindIII, MspI, NruI, PstI, SstI, StyII, XbaI, and XhoI (16 unrelated individuals). |
| References： | Bloch KD, et al. Structural organization and chromosomal assignment of the gene encoding endothelin. J. Biol. Chem. 264: 10851-10857, 1989. PubMed: 2659594 Hoehe MR, et al. TaqI identifies a four allele DNA polymorphism of the human endothelin-1 gene (EDN1). Nucleic Acids Res. 19: 3161, 1991. PubMed: 2057379 Kenneth D Bloch, personal communication |