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| 产品名称： | pUR18 |
|---|---|
| 商品货号： | TS132553 |
| Designations： | pUR18 |
| Depositors： | NC Barbet, AM Carr |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5000000000000000 Vector: pUR18 (plasmid) Promoters: Promoter lac Construction: pUC18, pON163 Marker(s):ampR,ura4+ Construct size (kb): 5.5 Features: insert detection: lacZ marker(s): ampR marker(s): ura4+ promoter: lac replicon: ars1 replicon: pMB1 |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker. The order of the major features in this plasmid is: ars1 - ura4 - HindIII/MCS/EcoRI/lacZ - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485 |
| Related Products： | component of:ATCC 87686 |
| Shipped： | frozen |
| Shipping Information： | Freeze dried E. coli containing the plasmid |