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| 产品名称： | p30-1-60 |
|---|---|
| 商品货号： | TS132644 |
| Designations： | p30-1-60 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Vector： | Construct size (kb): 4.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 1.0 Gene product: DNA Segment, single copy D3S86 Alleles: A1, A2 |
| Insert Size (kb)： | 1.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; EcoRI/HindIII--3.1, 1.2; EcoRI/PstI--3.1, 1.2; SmaI--4.1; HindIII--4.0. D3S86 has been mapped to 3p on the human chromosome. Final stringency wash is with 0.1X SSC/0.1% SDS at 50-55C. At higher temperatures, most of the probe is washed off. Enzyme(s) not detecting polymorphism: TaqI, MspI. |
| References： | Litt M, et al. An anonymous single-copy clone, p30-1-60, identifies a frequent RFLP on chromosome 3p HGM9 No. D3S86. Nucleic Acids Res. 17: 2883, 1989. PubMed: 2566158 |