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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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lambdaA6 [pcD-GALT]

货号 TS132650
中文名称 null
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产品名称: lambdaA6 pcD-GALT
商品货号: TS132650
Designations: lambdaA6 pcD-GALT
GenBank Number:

X07208

Species: Homo sapiens, human
Vector:
Construct size (kb): 45.5
Insert:
DNA: genomic
Insert lengths(kb): 16.0
Tissue: AT5B1 line (T-cell chronic lymphocytic leukemia)
Gene product: T cell receptor, gamma, variable region V9 TCRGC1
Alleles: A2, A1
Insert Size (kb): 16.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): SacI--20.0, 6.3, 0.9; SmaI--19.0, 9.2, 6.0, 5.2, 4.6; XbaI--20.0, 12.0, 9.1, 3.2; XhoI--20.0, 16.0, 9.1; KpnI--17.0, 15.0, 7.2, 4.0, 1.5.
The insert contains the following restriction sites (approximate kb from the 5 end): SacI--6.3, 15.3, 16 (polylinker site).
Contains the pseudogene VgammaA, a non-productive Vgamma9-Jgamma1 rearrangement, and exon 1 of TCRGC1. The 5 end begins about 8 kb upstream of Vgamma9 and extends about 7 kb beyond J1.
Additional GenBank/EMBL accession numbers: X08086, M12957.
References:

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication