产品名称: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
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商品货号: | TS132728 |
Deposited As: | Saccharomyces cerevisiae Hansen, teleomorph |
Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
Strain Designations: | YPH98 |
Alternate State: | Candida robusta Diddens et Lodder |
Application: | Host strains for transformation, P. Hieter strains (BA1) |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format: | frozen |
Type Strain: | no |
Genotype: | MATa ura3-52 lys2-801(amber mutation) ade2-101(ochre mutation) leu2-delta1 trp1-delta1
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Preceptrol®: | no |
Mating Type: | a |
Ploidy: | haploid |
Comments: | Host strains for transformation, P. Hieter strains (BA1) |
Medium: | ATCC® Medium 1069: YPAD medium |
Growth Conditions: | Temperature: 25.0°C Atmosphere: Typical aerobic |
Name of Depositor: | YGSC |
Special Collection: | Yeast Genetic Stock Center |
Chain of Custody: | ATCC < |
References: | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ . Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ . |