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微信小章| 产品名称： | CHLA-02-ATRT |
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| 商品货号： | TS132769 |
| Organism： | Homo sapiens, human |
| Tissue： | brain/brain tumor |
| Product Format： | frozen |
| Morphology： | rounded |
| Culture Properties： | single cells and clusters in suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Atypical Teratoid Rhabdoid Tumor (ATRT) |
| Age： | 1.7 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | CHLA-02-ATRT was established from a 1 year old boy with atypical teratoid rhabdoid tumor (ATRT). Cells were isolated from the resected brain tumor at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. |
| Comments： | Diagnosis of ATRT was validated by immunohistochemistry that demonstrated negative expression of INI-1 in the tumor cells, but normal expression by endothelium on the same section.xa0 This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes.xa0 The generation of this cell line was made possible with the support of Graysons Fund and the Michael Hoefflin Foundation for Childrens Cancer to Childrens Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community. |
| Complete Growth Medium： | DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium at 2 X 105 to 3 X 105 viable cells/mL. Do not allow the cell concentration to exceed 2 X 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended. Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Freeze medium: fetal bovine serum, 50%; Cell Freezing Medium-DMSO (Sigma, Cat. No. C-6295), 50% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: XY CSF1PO: 11,10 D13S317: 11,13 D16S539: 13 D5S818: 11,12 D7S820: 10,12 THO1: 6 TPOX: 10,12 vWA: 16,18 |
| Name of Depositor： | Anat Erdreich-Epstein, Saban Research institute, Childrens Hospital Los Angeles |