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| 产品名称： | pHP45omega-Tc JF297 |
|---|---|
| 商品货号： | TS132790 |
| Designations： | pHP45omega-Tc JF297 |
| Depositors： | J Frey |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4800000190734860 Vector: pHP45omega-Tc (plasmid) Construction: pHP45:omega, pBR322 tetR Marker(s):ampR,tetR Construct size (kb): 4.480000019073486 Features: marker(s): ampR, tetR replicon: pMB1 terminator: T4D gene 32 |
| Applications： | integrating vector shuttle vector vector permitting positive selection for integration vector useful for interposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.5, 2.4; HindIII--2.45, 2.3; SalI--4.9. Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate. One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative genera. pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-ended 1.4 kb HindIII/AvaI fragment of pBR322 containing the tetracycline resistance gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679 Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930 |
| Shipped： | freeze-dried |