产品名称: | pPAI75.1 |
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商品货号: | TS132793 |
Designations: | pPAI75.1 |
GenBank Number: | J02685 |
Species: | Homo sapiens, human |
Depositors: | JE Sadler |
Vector: | Construct size (kb): 4.599999904632568 |
Insert: | DNA: cDNA Insert lengths(kb): 1.899999976158142 Tissue: placenta Gene product: plasminogen activator inhibitor, type II (arginine-serpin) PAI2 Alleles: A1, A2, A1, A2 |
Insert Size (kb): | 1.900 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | The insert contains the following sites separated by (bp)(approx): SalI- 280- PvuII- 60- PstI- 50- NsiI- 90- SspI- 320- XbaI- 20- NdeI- 420- NsiI- 560- XbaI- 85- SalI. Restriction digests of the clone give the following sizes (kb): SalI--2.65, 2.0; EcoRI--2.65, 1.95; BamHI--4.65; HindIII--4.65; PstI--4.0, 0.59. This clone includes 55 nt of 5-noncoding sequence with a stop codon in-frame at residue -27, 1245 nt of open reading frame, a stop codon, 581 nt of 3-noncoding sequence and a poly(A) tail. The probe detects a single band of 2.0 kb in total mRNA from human placenta. |
References: | Ye RD, et al. cDNA cloning and expression in Escherichia coli of a plasminogen activator inhibitor from human placenta. J. Biol. Chem. 262: 3718-3725, 1987. PubMed: 3029122 |