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| 产品名称： | M2371 HIS3 --> ADE2 converter |
|---|---|
| 商品货号： | TS132799 |
| Designations： | M2371 HIS3 --> ADE2 converter |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.7340002059936520 Vector: M2371 (plasmid) Construction: pUC18 Marker(s):ampR ADE2 Construct size (kb): 7.734000205993652 Features: gene disruption cassette: his3::ADE2 marker(s): ampR replicon: pMB1 restriction site: BamHI...EcoRI restriction site: HindIII...BamHI |
| Applications： | YI-type (integrating) shuttle vector host modification marker swap vector HIS3 --> ADE2 |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 2.7, 0.7, 0.6; EcoRI--6.8, 0.9; PvuI--6.8, 0.9; HindIII--3.9, 1.6, 1.2, 1.0. To convert the host phenotype from HIS3 to ADE2, transform with the PstI+SacI digested vector (4.99 kb) and select for Ade+ transformants. A marker swap (HIS3->ADE2) vector for altering a Saccharomyce s cerevisiae host by a one-step gene disruption. When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. Vector was constructed by replacing an internal MscI-NsiI fragment of HIS3 with a 3.7 kb NotI (blunt)-PstI fragment containing the ADE2 coding sequence. ADE2 and HIS3 are in the opposite orientation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Macatee T, et al. Global alterations in chromatin accessibility associated with loss of SIN4 function.. Nucleic Acids Res. 25(6): 1240-1247, 1997. PubMed: 9092635 |
| Related Products： | component of:ATCC 87561 |
| Shipped： | freeze-dried |