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| 产品名称： | YIp353 |
|---|---|
| 商品货号： | TS132803 |
| Designations： | YIp353 |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0999999046325680 Vector: YIp353 (plasmid) Promoters: Promoter none Construction: YEp353 (ATCC 37725), YIp352 Marker(s):URA3,ampR Construct size (kb): 7.099999904632568 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: none replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2. Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1. One of 3 promoter-cloning, YI type shuttle vectors (TS132803 - 37745) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5 GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried xa0E. coli containing the plasmid |