One band appears above that for chromosome XI and the other appears very close to the band for chromosome IV. When used in conjunction with either strain STX454-1A ( ATCC 204649) or STX454-2A (ATCC 204651) in an OFAGE or CHEF gel and probed with the appropriate sequences, one is able to localize genes on any of the 16 chromosomes of yeast.

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Mapping strains: mapping by chromosome separation (AB10)

STX454-1B (TS132818) and STX454-2B (ATCC 204650) clearly resolve the doublet band No. 5 in an OFAGE or CHEF gel which encompasses chromosomes V and VIII of strain X2180-1A (ATCC 204647) or X2180-1B (ATCC 204655). They also resolve the two doublet bands 10 and 11 of OFAGE gels into single bands for chromosomes XIII (designated as chromosome XVI in previous catalogs, but identified by Southern blotting as chromosome XIII) and VII respectively. There is in these strains a reciprocal translocation, originating in one of the parents of the STX454- strains, X2181-1B (ATCC 204822), between chromosomes XVI and XV resulting in two new composite bands, each having a portion of each of these two chromosomes. One band appears above that for chromosome XI and the other appears very close to the band for chromosome IV. When used in conjunction with either strain STX454-1A ( ATCC 204649) or STX454-2A (ATCC 204651) in an OFAGE or CHEF gel and probed with the appropriate sequences, one is able to localize genes on any of the 16 chromosomes of yeast. The translocation involves a break at the ade2 locus on chromosome XV; the exact position of the translocation break point on chromosome XVI has not been determined, but it is very close to the centromere.

progeny of ATCC 204822 and ATCC 204626

Temperature: 25.0°C

Atmosphere: Typical aerobic

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derived from a cross of X2181-1B (ATCC 204822) x A364A (ATCC 204626)