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| 产品名称： | pMA200U |
|---|---|
| 商品货号： | TS132838 |
| Designations： | pMA200U |
| Depositors： | NE Dixon |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.1519999504089360 Vector: pMA200U (phagemid) Promoters: Promoter lambda PL Construction: pCE30 (ATCC 37830), pTZ18U Marker(s):ampR Construct size (kb): 4.151999950408936 Features: marker(s): ampR promoter: lambda PL, lambda PR replicon: pMB1, f1 repressor gene: cI857 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.3; BamHI/EcoRI--4.3; BglI--3.0, 1.3. Upon infection with M13KO7 (ATCC 37468), an M13-sensitive host strain containing a pMA200U derivative produces packaged single-stranded plasmid DNA complementary to the M13 universal primer. If the insert is oriented correctly for transcription from the lambda promoters, the single-stranded plasmid DNA will contain the coding strand. Expression vector encoding cI857. Contains primer sites useful for sequencing and permits production of ssDNA. Constructed by replacing the 1.118 kb BglI fragment of pCE30 with the analogous 1.27 kb fragment of pTZ18U. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Elvin CM, et al. Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 87: 123-126, 1990. PubMed: 2139621 |
| Shipped： | freeze-dried |