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| 产品名称： | pPT150 |
|---|---|
| 商品货号： | TS132866 |
| Designations： | pPT150 |
| Depositors： | NE Dixon |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4050002098083500 Vector: pPT150 (plasmid) Promoters: Promoter lambda PL Construction: pND201 (ATCC 37831), pBR322 Marker(s):ampR Construct size (kb): 4.40500020980835 Features: marker(s): ampR promoter: lambda PL, lambda PR replicon: pMB1 repressor gene: cI857 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; BamHI/EcoRI--4.2, 0.4; BglI--3.0, 1.2, 0.40. Inserts containing up to 350 bp upstream of the start codon can be trimmed with Bal31 after linearizing with an enzyme cutting upstream of the insert. Recircularize the vector to bring a start codon into juxtaposition with a strong ribosome-binding site. Because cI857 is expressed by the plasmid itself, from its natural promoter PM, the vector may be used in virtually any E. coli strain. The sequence surrounding the cloning sites has been used to design primers for direct sequencing, including the M13 universal primer. Expression vector containing a strong ribosome-binding site and primer sites useful for sequencing. Encodes cI857. Constructed by inserting a segment of pBR322 (nt 975 - nt 1338) between the HpaI site and a cluster of restriction sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Elvin CM, et al. Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 87: 123-126, 1990. PubMed: 2139621 |
| Shipped： | freeze-dried |