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| 产品名称： | pJEL144 |
|---|---|
| 商品货号： | TS132890 |
| Designations： | pJEL144 |
| Depositors： | P Valentin-Hansen |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.6999998092651400 Vector: pJEL144 (plasmid) Promoters: Promoter deoP2 Construction: pJEL170, pJEL132 Marker(s):ampR Construct size (kb): 12.69999980926514 Features: insert detection: lacZ marker(s): ampR promoter: deoP2 replicon: R1 repressor gene: cI857 terminator: none |
| Applications： | terminator-cloning vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--13.6; EcoRI--13.6. Transcription from deoP2 is negatively regulated by the DeoR and CytR repressors and depends on the cAMP/CRP complex. Below 37C, there is one copy per genome equivalent stabilized by the parB locus. At 42C replication is uncontrolled because induction of the cI857-regulated PR promoter permits expression of the copB, copA, and repA genes. Expression of beta-galactosidase from this vector can be increased by the use of hosts containing repressor mutations such as S0929 (cytR), S0930 (deoR) and S0931 (cytR, deoR). Terminator-testing plasmid. Termination signal-containing sequences cloned into the BamHI site may reduce expression of beta-galactosidase activity regulated by the deoP2 promoter. Constructed by inserting a 0.22 kb EcoRI/BamHI fragment containing the deoP2 regulatory region and the first 16 codons of deoC from pJEL134 into pJEL170. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Larsen JE, et al. Analysis of the terminator region after the deoCABD operon of Escherichia coli K-12 a new class of single copy number operon-fusion vectors. Nucleic Acids Res. 15: 5125-5140, 1987. PubMed: 3299264 |
| Shipped： | freeze-dried |