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| 产品名称： | pUR278-183K |
|---|---|
| 商品货号： | TS133009 |
| Designations： | pUR278-183K |
| Species： | Tobacco mosaic virus |
| Depositors： | WO Dawson |
| Applications： | produces protein 183K protein |
| Vector： | Construct size (kb): 6.329999923706055 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.129999995231628 Gene product: 183K protein Target Gene: 183K protein |
| Insert Size (kb)： | 1.130 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--5.6, 0.8; EcoRI--5.6, 0.8, 0.5; HindIII--6.8; BamHI/ClaI--3.3, 2.3, 1.25; BamHI--6.8; ClaI--3.6, 3.2. This clone expresses an antigen specific for the 183K protein of tobacco mosaic virus. A fragment containing the 183K gene (nt 3572 to approx. 4700) of pTMV204 was ligated into pUR278. |
| Classification： | Tobamovirus |
| References： | Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986. Lehto K, et al. Time course of TMV 30K protein accumulation in intact leaves. Virology 174: 290-293, 1990. PubMed: 2294644 William O Dawson, personal communication |