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| 产品名称： | HHCMG75 |
|---|---|
| 商品货号： | TS133011 |
| Designations： | HHCMG75 |
| GenBank Number： | M78038 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.900000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D1S282E Genomic copy number: unique Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: U Sequence homology: Homo sapiens agrin Chromosome: 1; Localization: 1 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.1 Cross references: DNA Seq. Acc.: M78038; EST: EST01625; Primer: M13 Forward DNA Seq. Acc.: M78829; EST: EST00977; Primer: M13 Reverse Insert lengths(kb): 2.099999904632568 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: agrin AGR* |
| Insert Size (kb)： | 2.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; PstI--3.8, 1.1; BamHI--4.8. According to the coding-region prediction program CRM, these sequence ranges (followed by the probability) of EST00977 may contain coding sequence: 182-141 (good). EST01625 matches GenBank accession RATAGR with 84.60% identity over the sequence range: 10-113. This sequence record is for rat agrin. |
| References： | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586 J C Venter, personal communication |