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| 产品名称： | IB2329 EST41698, SGC32142 |
|---|---|
| 商品货号： | TS133054 |
| Designations： | IB2329 EST41698, SGC32142 |
| GenBank Number： | T15978 |
| Species： | Homo sapiens, human |
| Depositors： | JM Sikela |
| Applications： | The primers can also be used to generate sequence from the 3 or 5 ends, respectively. |
| Vector： | Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene name: DNA Segment, expressed Contains complete coding sequence?: U Chromosome: 11; Localization: 11, 136.389999 cR Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: Modification: HindIII Insert 3 end: Modification: NotI Insert size (kb): 0.9 Cross references: DNA Seq. Acc.: T15978 Insert lengths(kb): 0.8999999761581421 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: DNA Segment, expressed |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.3, 0.94; NotI--4.5; HindIII/NotI--3.4, 0.92; PstI--4.5; EcoRI--4.5. Derived from a directionally cloned library with an average insert size of 1.7 kb. Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3 or 5 ends, respectively. |
| References： | Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745 Marcelo B Soares, personal communication |