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| 产品名称： | lambda27 GC.27 |
|---|---|
| 商品货号： | TS133100 |
| Designations： | lambda27 GC.27 |
| GenBank Number： | L10641 |
| Species： | Homo sapiens, human |
| Depositors： | A Dugaiczyk |
| Vector： | Construct size (kb): 49.79999923706055 DESCRIPTION OF VECTOR COMPONENT: Name of vector: Charon 4A Intact vector size: 46.000 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none promoter: none replicon: lambda terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GC Genomic copy number: unique Gene name: group-specific component (vitamin D binding protein) Contains complete coding sequence?: N Chromosome: 4; Localization: 4 q12-q13 Tissue: fetal liver (ATCC 37333) Type of DNA: genomic Insert end: HaeIII or AluI Modification: EcoRI linker Insert end: HaeIII or AluI Modification: EcoRI linker Insert size (kb): 11.8 Cross references: DNA Seq. Acc.: L10641 Nucleotides ~4900-16900 of the insert correspond to nucleotides ~1-12000 of L10641. Insert lengths(kb): 11.80000019073486 Tissue: fetal liver (ATCC 37333) Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC |
| Insert Size (kb)： | 11.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--22.0, 10.0, 4.5, 2.5, 1.8, 1.7, 0.7, 0.6; HindIII-->23, 18, 6.7; BglII--20, 7.2, 5.8, 4.85, 3.9, 2.8, 2.15, 1.7, 0.9, 0.65, 0.36; BamHI-->23, 3.9, 0.5; SacI-->23, 8.8, 4.7, 3.9, 1 TS133100/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI1 --2.5, 4.3, 4.9, 9.4, 10.1, 11.8; HindIII--1.1, 7.8; SacI--1.8, 3.3, 4.4. Begins in the 5 flanking region and continues into intron 1. Contains L1 (Kpn) repeats. |
| References： | Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650 |