宁波泰斯拓生物

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Rosculus sp.

货号 TS133116
中文名称 null
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产品简介
购买须知
产品名称: Rosculus sp.
商品货号: TS133116
Strain Designations: BSA-02190017
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
sediment, saltwater
Virginia, United States
Product Format: frozen
Storage Conditions: liquid nitrogen vapor phase
Type Strain: no
Medium: ATCC® Medium 1525: Seawater 802 medium
Growth Conditions:
Temperature: 20.0°C
Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831) and as a food source
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Subcultivation:
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Cryopreservation:
Storage temperature: liquid nitrogen vapor phase
Name of Depositor: JC Cole, TA Nerad
Special Collection: NSF - Protistology
Geographical Isolation: Cattleshed Marsh (near Hog Island)
References:

sampled from a sparina area; Catleshed Marsh (near Hog Island), eastern shore of Virginia