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| 产品名称： | HFBCE92 |
|---|---|
| 商品货号： | TS133125 |
| Designations： | HFBCE92 |
| GenBank Number： | M77961 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SMPD1 Genomic copy number: unique Gene name: sphingomyelin phosphodiesterase 1, acid lysosomal Contains complete coding sequence?: U Chromosome: 11; Localization: 11 p15.3 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.6 Cross references: DNA Seq. Acc.: M77961; EST: EST01545; Primer: M13 Forward Insert lengths(kb): 1.600000023841858 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: sphingomyelin phosphodiesterase 1, acid lysosomal SMPD1 |
| Insert Size (kb)： | 1.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.6; BamHI--3.8, 0.8; PstI--4.6. EST01545 matches GenBank accession HUMASM2MR with 97.5% identity over the sequence range: 218-59. |
| References： | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 J C Venter, personal communication |