| 产品名称： |
Paramecium octaurelia Sonneborn |
| 商品货号： |
TS133130 |
| Strain Designations： |
stock 299 Lambda ($ 299 lambda) |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： |
Barro Colorado Island, Panama, 1957 |
| Product Format： |
test tube |
| Storage Conditions： |
Test Tube: See handling procedure |
| Axenic/Xenic： |
Xenic |
| Type Strain： |
no |
| Comments： |
carries the type strain of the bacterial endosymbiont Lyticum flagellatum Preer et al. |
| Medium： |
ATCC® Medium 802: Sonneborns Paramecium medium
|
| Growth Conditions： |
Temperature: 25°C |
| Cryopreservation： |
Cryoprotective Solution
DMSO, 1.5 ml
Fresh growth medium w/o bacteria, 7.5 ml
MgCl2 (0.5 mM), 0.5 ml
CaCl2 (0.5 mM), 0.5 ml
- Mix the components in the order listed.xa0 Before adding the MgCl2 and the CaCl2 allow the solution to return to room temperature.xa0 When the medium is added to the DMSO the solution will warm up due to chemical heat.
- Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 200 x g for 1 min.
- Adjust the concentration of cells to 2 x 105/ml in fresh medium.
- Mix the cell preparation and the cryoprotective solution in equal portions.
- Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
- Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
- Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
- To establish a culture from the frozen state add 1.0 ml ATCC medium 802 to the frozen ampule and place it in a 35°C water bath.xa0 Immerse the vialxa0 to a level just above the surface of the frozen material. Do not agitate the vial.
- xa0Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 ml of bacterized ATCC medium 802.
- Incubate at 25°C.
- Once the culture is established, transfer 0.5 ml to 5.0 ml of bacterized ATCC medium 802.
- Follow the protocol for maintenance of culture.
|
| Name of Depositor： |
JR Preer |
| Chain of Custody： |
ATCC <-- JR Preer <-- T. Sonneborn <-- D. Sonneborn |
| Year of Origin： |
1957 |
| References： |
Preer JR, Preer LB. Revival of names of protozoan endosymbionts and proposal of Holospora caryophila nom. nov.. Int. J. Syst. Bacteriol. 32: 140-141, 1982.
Proc. Indiana Acad. Sci. 67: 302, 1958.
|
微信小陈
微信小章