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| 产品名称： | HFBEF62 |
|---|---|
| 商品货号： | TS133140 |
| Designations： | HFBEF62 |
| GenBank Number： | T07227 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.75 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: UCHL1 Gene name: ubiquitin carboxyl-terminal esterase L1 Contains complete coding sequence?: N Chromosome: 4; Localization: 4 p14 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 0.76 Cross references: DNA Seq. Acc.: T07227; EST: EST05116; Primer: M13-21; THC id: THC100992 DNA Seq. Acc.: X04741; EST: EST05116 Nucleotides 23-279, 275-330 of the insert correspond to nucleotides 985-729, 734-679 of X04741 with 97% identity. Insert lengths(kb): 0.7599999904632568 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: ubiquitin carboxyl-terminal esterase L1(ubiquitin carboxyl-terminal hydrolase PGP9.5) UCHL1 |
| Insert Size (kb)： | 0.760 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.41, 0.35. The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. |
| References： | Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434 |