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| 产品名称： | pfXblue |
|---|---|
| 商品货号： | TS133161 |
| Designations： | pfXblue |
| Depositors： | ME Brandt |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.6270000934600830 Vector: pfXblue (plasmid) Promoters: Promoter lambda PL Construction: pMb3, M13mp18, factor Xa Marker(s):ampR Construct size (kb): 3.627000093460083 Features: insert detection: lacZ marker(s): ampR promoter: lambda PL replicon: pMB1 |
| Applications： | expression vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants vector useful for cloning PCR products |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.8 (doublet); EcoRI--3.6; PstI--2.1, 1.55. Restriction digests of the plasmid give the following sizes (kb): BamHI--3.574, 0.053; BglI--1.843, 1.784; PstI--2.072, 1.555; PvuI--2.063, 1.564; PvuII--2.972, 0.655; SacI--3.257, 0.37. Inserts can be cloned using the 5 StuI site and one other MCS restriction site matching the 3 end of the insert. The vector supplies the AUG initiator codon. The insert to be cloned should have a phosphorylated 5 blunt end with the first base corresponding to the first base of the first codon. Expression vector permitting the production of a fusion protein that can be trimmed by cleavage with the coagulation factor Xa protease. A modified lacZ allows blue/white screening of colonies. Especially useful for the expression of PCR products. The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - lambda cII - factor Xa recognition sequence - StuI/MCS/HindIII/modified lacZ. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brandt ME, et al. A vector for directional cloning and expression of polymerase chain reaction products in Escherichia coli. Gene 97: 113-117, 1991. PubMed: 1995421 Mark E Brandt, personal communication |
| Shipped： | freeze-dried |