产品名称: | pUNG40 |
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商品货号: | TS133175 |
Designations: | pUNG40 |
GenBank Number: | X15653 |
Species: | Homo sapiens, human |
Applications: | It does not include the complete coding region, but it does include the complete region which is conserved among uracil-DNA glycosylases. |
Vector: | Construct size (kb): 4.735000133514404 |
Insert: | DNA: cDNA Insert lengths(kb): 1.726999998092651 Tissue: placenta Gene product: uracil-DNA glycosylase(uracil DNA glycosylase) DGU |
Insert Size (kb): | 1.727 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.5, 0.2; EcoRI--3.0, 1.7; XbaI--4.7; HindIII--3.8, 0.9; XhoI--4.7. The insert contains the following restriction sites (nt from 5 endnt according to published sequence): BamHI--15, 256 297, 538; SacII--33 315; StuI--308 590; NcoI--388 670; HindIII--850 1132. Gives a strong hybridization signal with genomic EcoRI fragments of 7 and 3.5 kb. Extends from nt 283 to nt 2005 of the published sequence. It does not include the complete coding region, but it does include the complete region which is conserved among uracil-DNA glycosylases. Oriented such that transcription from the T7 promoter gives the sense strand. |
References: | Olsen LC, et al. Molecular cloning of human uracil-DNA glycosylase, a highly conserved DNA repair enzyme*. EMBO J. 8: 3121-3125, 1989. PubMed: 2555154 Aasland R, et al. Chromosomal assignment of human uracil-DNA glycosylase to chromosome 12. Genomics 7: 139-141, 1990. PubMed: 2335354 Lisbeth C Olsen, personal communication |