产品名称: | pcDGALT pcD-GALT |
---|---|
商品货号: | TS133191 |
Designations: | pcDGALT pcD-GALT |
GenBank Number: | M18731 |
Species: | Homo sapiens, human |
Applications: | in another host, produces protein galactose-1-phosphate uridylyltransferase |
Vector: | Construct size (kb): 4.400000095367432 |
Insert: | DNA: cDNA Insert lengths(kb): 1.399999976158142 Gene product: galactose-1-phosphate uridylyltransferase GALT Target Gene: galactose-1-phosphate uridylyltransferase |
Insert Size (kb): | 1.400 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.4; HindIII--4.5; PstI--2.1, 1.3, 0.8, 0.2; PvuII--4.5; XhoI--3.0, 1.0, 0.6. The insert contains the following restriction sites (nt position according to the revised sequence): BamHI--12; PstI--141, 336, 1093; XhoI--393; PvuII--979. Detects genomic EcoRI fragments of the following sizes (kb): human--12.0; Chinese hamster (CHO-K1 cell line)--9.0. When transfected into COS-7 cells or human cells from a galactosemic individual (GM639), enzyme activity was expressed. |
References: | Flach JE, et al. Sequence of a cDNA encoding human galactose-1-phosphate uridyl transferase. Mol. Biol. Med. 7: 365-369, 1990. PubMed: 2233247 Reichardt JK, Berg P. Cloning and characterization of a cDNA encoding galactose-1-phosphate uridyl transferase. Mol. Biol. Med. 5: 107-122, 1988. PubMed: 2840550 |