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| 产品名称： | pSP417 |
|---|---|
| 商品货号： | TS133278 |
| Designations： | pSP417 |
| Depositors： | SM Podkovyrov |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.1999998092651370 Vector: pSP417 (plasmid) Construction: pRS415 Marker(s):ampR Construct size (kb): 9.199999809265137 Features: marker(s): ampR replicon: pMB1 reporter group: lacZ MCS: EcoRI...BamHI transcription terminator: T1 coding sequence: lacY |
| Applications： | promoter-cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.2; BglII--9.2; SalI--9.2; NotI--9.2; PvuI--6.2, 1.8, 0.75, 0.45. Distributed in E. coli strain SP2 (ATCC 47089) which contains a pcnB mutation that limits pMB1-based plasmid copy number to approximately 10% of wild type. This is useful where high copy number may interfere with the fusion analysis. A high copy number, promoter-cloning plasmid that with very low levels of background lacZ expression. Permits detection of weak transcription signals. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Simons RW, et al. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96, 1987. PubMed: 3596251 Podkovyrov SM, Larson TJ. A new vector-host system for construction of lacZ transcriptional fusions where only low-level gene expression is desirable. Gene 156: 151-152, 1995. PubMed: 7737510 |
| Shipped： | freeze-dried |