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| 产品名称： | pH9RA3 |
|---|---|
| 商品货号： | TS133391 |
| Designations： | pH9RA3 |
| GenBank Number： | M84747 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein interleukin 9 receptor |
| Vector： | Construct size (kb): 7.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pRc/RSV Intact vector size: 5.100 Type of vector: phagemid Vector end: BstXI Vector end: BstXI Cloning sites: HindIII SpeI BstXI NotI XbaI Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI NsiI XbaI Construction: pUC19, RSV, bovine growth hormone Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: RSV LTR replicon: pMB1, M13 terminator: BGH polyadenylation Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IL9R Gene name: interleukin 9 receptor Contains complete coding sequence?: Y Tissue: megakaryoblastic leukemia Mo7E cell line Type of DNA: cDNA Insert end: BstXI Insert end: BstXI Insert size (kb): 2.3 Cross references: DNA Seq. Acc.: M84747 Insert lengths(kb): 2.299999952316284 Tissue: megakaryoblastic leukemia Mo7E cell line Gene product: interleukin 9 receptor IL9R Target Gene: interleukin 9 receptor |
| Insert Size (kb)： | 2.300 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/NotI--5.4, 2.5; BamHI--3.0, 2.85, 2.1; PstI--6.4, 1.5; SmaI--5.2, 2.75; EcoRI--4.4, 3.5. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.08, XbaI--0.33; SfiI--1.17; SmaI--1.33; SacI--1.34, 1.57, 2.25. Contains the complete coding sequence (nt 180-1748) plus 5 and 3 untranslated regions. The 5 end of the insert is adjacent to the unique HindIII and SpeI sites of the MCS; the 3 end of the insert is adjacent to the unique NotI site of the MCS. |
| References： | Renauld JC, et al. Expression cloning of the murine and human interleukin 9 receptor cDNAs. Proc. Natl. Acad. Sci. USA 89: 5690-5694, 1992. PubMed: 1376929 Jean-Christophe Renauld, personal communication |