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| 产品名称： | lambda93 GC.93 |
|---|---|
| 商品货号： | TS133419 |
| Designations： | lambda93 GC.93 |
| GenBank Number： | L10641 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 51.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: Charon 4A Intact vector size: 46.000 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none promoter: none replicon: lambda terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GC Genomic copy number: unique Gene name: group-specific component (vitamin D binding protein) Contains complete coding sequence?: N Chromosome: 4; Localization: 4 q12-q13 Tissue: fetal liver (ATCC 37333) Type of DNA: genomic Insert end: HaeIII or AluI Modification: EcoRI linker Insert end: HaeIII or AluI Modification: EcoRI linker Insert size (kb): 19.95 Cross references: DNA Seq. Acc.: L10641 Nucleotides ~1-19950 of the insert correspond to nucleotides ~6300-26300 of L10641. Insert lengths(kb): 19.95000076293945 Tissue: fetal liver (ATCC 37333) Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC |
| Insert Size (kb)： | 19.950 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11.0, 5.0, 3.7, 2.6, 2.55, 1.9, 1.8, 0.95, 0.7; HindIII-->23, 5.9; BamHI-->23, 7.3, 5.7, 4 (doublet), 1.6; BglII--5.5, 5.1, 4.9 (doublet), 3.5, 3.1, 2.75, 2.5, 2.15, 2.0, 1.8 (doublet), 1.7, 1.55 (doublet), 1.4, 0.65; SstI-->23, 9.0, 4.1, 3.9, 2.8, 1.9. ATCC 95662/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--3.3, 4.0, 5.7, 9.4, 12.0, 12.6, 14.4, 14.55, 16.45, 19; HindIII--1.6; SacI--9, 11.8, 15.9. Begins in intron 1 and continues into intron 6. ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5. Contains L1 (Kpn) and Alu repeats. |
| References： | Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650 |