产品名称: | pYNH24 |
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商品货号: | TS133497 |
Designations: | pYNH24 |
Species: | Homo sapiens, human |
Depositors: | RL White, Y Nakamura |
Vector: | Construct size (kb): 4.900000095367432 |
Insert: | DNA: genomic Insert lengths(kb): 2.0 Gene product: DNA Segment, single copy D2S44 |
Insert Size (kb): | 2.0 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; HindIII--4.6; EcoRI/HindIII--2.5, 1.95; PvuII--2.15, 1.53, 0.76; BglI--3.6, 1.08. MspI fragment from cYNH24 cloned into AccI site of pUC18; RFLPs detected under normal hybridization stringency; washes at 0.1X SSC, 0.1% SDS, 65 C. Enzyme(s) not detecting polymorphism: RsaI. |
References: | Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44). Nucleic Acids Res. 15: 10073, 1987. PubMed: 2892167 |