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微信小章| 产品名称： | RK2-2 |
|---|---|
| 商品货号： | TS133542 |
| Designations： | RK2-2 |
| GenBank Number： | U07000 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 49.57699966430664 DESCRIPTION OF VECTOR COMPONENT: Name of vector: svPHEP Intact vector size: 9.201 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI ClaI Polylinker sites: HindIII ClaI EcoRI BamHI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L19900 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: BCR Other gene symbols: BCR1 Genomic copy number: unique Gene name: breakpoint cluster region Contains complete coding sequence?: N Chromosome: 22; Localization: 22 q11 Tissue: chronic myelogenous leukemia K-562 cell line Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 40.376 Cross references: DNA Seq. Acc.: U07000 Nucleotides 1-40376 of the insert correspond to nucleotides 53441-93816 of U07000. Insert lengths(kb): 40.37599945068359 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: breakpoint cluster region BCR Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 40.376 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 7.6, 6.3, 4.7, 3.45, 2.95, 2.2, 2.0, 1.4, 1.15, 0.52; BglII--13.5, 10.5, 9.4, 7.1, 4.05, 3.5, 2.85; EcoRI--10.0, 9.4, 8.6, 7.2, 6.6, 4.0, 3.5, 2.7; HindIII--17.5, 13.5, 8.6, 6.6, 1.7, 1.25, 0.66; SstI--8.8 (doublet), 7.6, 5.4, 4.7, 3.8, 2.15, 1.9, 1.7, 1.6, 1.45, 1.1, 0.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins in intron 3 and continues past exon 2 into intron 2. |
| References： | Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859 Heisterkamp N, et al. The first BCR gene intron contains breakpoints in Philadelphia chromosome positive leukemia. Nucleic Acids Res. 16: 10069-10081, 1988. PubMed: 3194194 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |