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| 产品名称： | pAX-7 |
|---|---|
| 商品货号： | TS133546 |
| Designations： | pAX-7 |
| Applications： | Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, EcoRV, HindIII, MspI, PstI, TaqI. Tested against the X chromosome of 40 Sardinian males. |
| Vector： | Construct size (kb): 4.75 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.049999952316284 Tissue: HeLa cell line Gene product: DNA Segment, numerous copies DNF3 Alleles: A2, A1 |
| Insert Size (kb)： | 1.050 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.7, 1.05, 0.25, 0.1; BamHI--4.7; EcoRI--4.7; AvaI--2.9, 1.7, 0.2. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The SstI polymorphism has been mapped to the X chromosome and has been studied in 15 families. The insert contains an internal PstI site which results in two fragments, 1000 bp pAX-7-1 and 300 bp pAX-7-2. For EcoRI digested human DNA, pAX-7-1 hybridizes with 4 fragments and pAX-7-2 hybridizes with only 2 fragments. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, EcoRV, HindIII, MspI, PstI, SacI, TaqI. For chromosomes 12, 18, and 21 of 15 Sardinian males. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, EcoRV, HindIII, MspI, PstI, TaqI. Tested against the X chromosome of 40 Sardinian males. |
| References： | Balazs I, et al. Isolation and characterization of human random cDNA clones homologous to DNA from the X chromosome. Somatic Cell Mol. Genet. 10: 385-397, 1984. PubMed: 6087473 Barbara Weiffenbach, personal communication |